|
KMID : 0545120200300010127
|
|
Journal of Microbiology and Biotechnology
2020 Volume.30 No. 1 p.127 ~ p.135
|
|
|
sRNA EsrE Is Transcriptionally Regulated by the Ferric Uptake Regulator Fur in Escherichia coli
|
|
Hou Bingbing
Yang Xichen
Xia Hui
Wu Haizhen
Ye Jiang
Zhang Huizhan
|
|
|
Abstract
|
|
|
|
Small RNAs (sRNAs) are widespread and play major roles in regulation circuits in bacteria. Previously, we have demonstrated that transcription of esrE is under the control of its own promoter. However, the regulatory elements involved in EsrE sRNA expression are still unknown. In this study, we found that different cis-regulatory elements exist in the promoter region of esrE. We then screened and analyzed seven potential corresponding trans-regulatory elements by using pull-down assays based on DNA affinity chromatography. Among these candidate regulators, we investigated the relationship between the ferric uptake regulator (Fur) and the EsrE sRNA. Electrophoresis mobility shift assays (EMSAs) and β-galactosidase activity assays demonstrated that Fur can bind to the promoter region of esrE, and positively regulate EsrE sRNA expression in the presence of Fe2+.
|
|
|
KEYWORD
|
|
|
Small RNA, EsrE, DNA affinity chromatography, Fur, transcriptional regulation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|